Analytica Chimica Acta, August 2022, 340288,

Long Mi ,Bing Lin ,Jianfeng Jin,Haiqing Zhang**, Hongwei Chen, Ziyi Cheng【程子译】,Jinsheng Wu ***, Heng Liu*【刘恒】

As a member of reactive sulfur molecules, hydrogen polysulfide (H2Sn) plays a vital role in cell protection, anti oxidative stress and regulation of redox signaling. The highly selective and sensitive detection of H2Sn was still  challenging due to its special nucleophilic and electrophilic reactivity. By incorporating phenyl 2-(benzoylthio)  benzoate into semi-naphthofluorescein, we developed a novel red emissive fluorescent probe SNAFL-H2Sn for the  detection of a representative H2Sn (e. g. H2S2). The addition of H2S2 would rapidly trigger SNAFL-H2Sn to  produce significant turn-on fluorescence signal changes at 626 nm with a linear response over a range of 2–30 μM  and a detection limit of 16 nM. SNAFL-H2Sn was capable of mapping exogenous and endogenous H2S2 in living  cells and zebrafish. Moreover, SNAFL-H2Sn was applied to detect endogenous H2S2 under atorvastatin stimula tion. The present study demonstrated that SNAFL-H2Sn potentially served as a promising tool for interrogating  H2Sn functions in biological systems.