Near-infrared II plasmonic porous cubic nanoshells for in vivo noninvasive SERS visualization of sub-millimeter microtumors

近红外II区等离子体多孔立方体纳米壳用于亚毫米级微肿瘤的体内无损伤SERS可视化

主讲人：赵晨霄

Nature Communications丨Received: 16 February 2022 丨Accepted: 25 August 2022 丨Published online: 06 September 2022 丨Page：1-11 丨DOI：https://doi.org/10.1038/s41467-022-32975-w

Abstract:

        In vivo surface-enhanced Raman scattering (SERS) imaging allows non-invasive visualization of tumors for intraoperative guidance and clinical diagnostics. However, the in vivo utility of SERS is greatly hampered by the strong optical scattering and autofluorescence background of biological tissues and the lack of highly active plasmonic nanostructures. Herein, we report a class of porous nanostructures comprising a cubic AuAg alloy nanoshell and numerous nanopores. Such porous nanostructures exhibit excellent near-infrared II plasmonic properties tunable in a broad spectral range by varying the pore features while maintaining a small dimension. We demonstrate their exceptional near-infrared II SERS performance varying with the porous properties. Additionally, near-infrared II SERS probes created with porous cubic AuAg nanoshells are demonstrated with remarkable capability for in vivo visualization of sub-millimeter microtumors in a living mouse model. Our near-infrared II SERS probes hold great potentials for precise demarcation of tumor margins and identification of microscopic tumors.

摘要：

  体内表面增强拉曼散射（SERS）成像可以无创地观察肿瘤，用于术中引导和临床诊断。 然而，由于生物组织的强光散射和自发荧光背景以及缺乏高活性的质子纳米结构，SERS的体内效用受到很大阻碍。在此，我们报告了一类多孔纳米结构，包括立方AuAg合金纳米壳和许多纳米孔。这类多孔纳米结构表现出优异的近红外II类等离子体特性，可通过改变孔隙特征而保持较小的尺寸在宽广的光谱范围内进行调节。我们证明了它们特殊的近红外II型SERS性能随着多孔性的变化而变化。此外，用多孔的立方体AuAg纳米壳制作的近红外II SERS探针在活体小鼠模型中对亚毫米的微肿瘤进行体内可视化的能力得到了证明。我们的近红外二代SERS探针在精确划分肿瘤边缘和识别微观肿瘤方面具有巨大的潜力。