Talanta, 2019, 197, 431-435Full text

Wangbo Qu ¹, Changhe Niu ², Xiaoyu Zhang ¹, Wei Chen ³, Fabiao Yu ⁴, Heng Liu ⁵, Xiuhua Zhang ¹, Shengfu Wang ¹

https://www.sciencedirect.com/science/article/abs/pii/S0039914019300645

Abstract

Peroxynitrite (ONOO^-) is an important reactive oxygen species (ROS), which can react with a variety of biologically active species and cause many diseases, such as cancer, neurodegenerative disorders. Herein, we develop a novel far-red fluorescent probe DCM-KA, which is equipping with α-ketoamide moiety caged DCM-NH₂. The probe exhibits fluorescence off-on response to ONOO^- over other biological interfering analytes by ONOO^--induced α-ketoamide deprotection reaction. More importantly, the probe is biocompatible and has been successfully utilized to visualize endogenous ONOO^- production in macrophage cell line J774A.1.