Biomaterials, 2015, 63, 93–101Full text

Yu, Fabiao;  Gao, Min;  Li, Meng;  Chen, Lingxin*

https://www.sciencedirect.com/science/article/abs/pii/S0142961215005153

Abstract

Intracellular reactive sulfur species play important roles in physiological and pathological processes. Emerging evidences suggest that sulfane sulfur instead of H₂S is the actual signaling molecule in these processes. Sulfane sulfur can be generated as a result of the reaction between O2·− and H₂S in mitochondria. Therefore, we develop a near-infrared mitochondria-targeting probe that allows multiresponse to O2·− and H₂S_n successively for investigating this biosynthetic reaction. The probe exhibits highly selective fluorescent response to O2·− and H₂S_nsuccessively in presence of potential biological interferants. Fluorescent imaging studies and flow cytometry analysis of RAW264.7 cells elaborate that the probe can be used to reveal the continuous process of O2·− burst and H₂S_n production in situ and in real-time. The mitochondria isolation indicates that the probe can specifically localize in mitochondria. Finally, the fluorescent probe has been successfully applied to detect O2·− and H₂S_n in mice.