Multifunctional nanoagents for ultrasensitive imaging and photoactive killing of Gram-negative and Gram-positive bacteria

用于革兰氏阴性和革兰氏阳性细菌的超灵敏成像和光活性杀灭的多功能纳米剂

主讲人：周艳虹

Nat Commun. 2019 Sep 6;10(1):4057.

Abstract:

Simultaneous imaging and treatment of infections remains a major challenge, with most current approaches being effective against only one specific group of bacteria or not being useful for diagnosis. Here we develop multifunctional nanoagents that can potentially be used for imaging and treatment of infections caused by diverse bacterial pathogens. The nanoagents are made of fluorescent silicon nanoparticles (SiNPs) functionalized with a glucose polymer (e.g., poly[4-O-(α-D-glucopyranosyl)-D-glucopyranose]) and loaded with chlorin e6 (Ce6). They are rapidly internalized into Gram-negative and Gram-positive bacteria by a mechanism dependent on an ATP-binding cassette (ABC) transporter pathway. The nanoagents can be used for imaging bacteria by tracking the green fluorescence of SiNPs and the red fluorescence of Ce6, allowing in vivo detection of as few as 105 colony-forming units. The nanoagents exhibit in vivo photodynamic antibacterial efficiencies of 98% against Staphylococcus aureus and 96% against Pseudomonas aeruginosa under 660 nm irradiation.

摘要：

同时对感染进行成像和治疗仍然是一个重大挑战，目前大多数方法只能对一组特定的细菌有效，或者对诊断没有帮助。在这里，我们开发了多功能纳米剂，可以潜在地用于成像和治疗由不同细菌病原体引起的感染。纳米试剂由荧光硅纳米颗粒(SiNPs)由葡萄糖聚合物(例如聚[4-O-(α- d -葡萄糖吡喃基)- d -葡萄糖吡喃糖])功能化，并负载氯e6 (Ce6)制成。它们通过依赖于atp结合盒(ABC)转运体途径的机制迅速内化到革兰氏阴性和革兰氏阳性细菌中。该纳米试剂可以通过跟踪SiNPs的绿色荧光和Ce6的红色荧光来成像细菌，允许在体内检测到少至105个集落形成单位。在660 nm照射下，纳米制剂对金黄色葡萄球菌的体内光动力抗菌效率为98%，对铜绿假单胞菌的体内光动力抗菌效率为96%。