Rational Design of Two-photon Fluorogenic Probe for VisualizingMonoamine Oxidase A Activity in Human Glioma 

Tissues

       人胶质瘤组织中单胺氧化酶A活性可视化双光子荧光探针的设计

主讲人：刘朝晖  

https://doi.org/10.1002/anie.202000059

Angew Chem Int Ed Engl. 2020 May 4;59(19):7536-7541.

Abstract: 

     Monoamine oxidases have two functionally distinct but  structurally similar isoforms (MAO-A and -B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO-A-specific two-photon fluorogenic probe (F1), capableof selective imaging of endogenous MAO-A enzymatic activities from a variety of biological samples, including MAO-A expressing neuronal SY-SY5Y cells, the brain of tumor-bearing mice and human Glioma tissues by using two-photon fluorescence microscopy (TPFM) with minimal cytotoxicity.

摘要：

单胺氧化酶有两种功能不同但结构相似的亚型(MAO-A 和 MAO-B)。通过使用荧光检测/成像技术来区分它们的能力具有重要的生物学意义，但是使用现有的化学工具进行区别还有很大的挑战性。这篇论文报道了 MAO-A 特异性双光子荧光探针F1，通过使用双光子荧光显微镜(TPFM)，能够选择性地对多种生物样品的内源性 MAO-A 酶活性进行成像，包括表达 MAO-A 的神经元 SY-SY5Y 细胞，荷瘤小鼠的脑和人胶质瘤组织，并且具有最小的细胞毒性。